Mouse acidic fibroblast growth factor 1 ELISA kit detection method - Database & Sql Blog Articles

Test - lowercase jpg

Kaixin micro test

Test probe P100-M3

Brand AVX TPSE226M035R0125 Low impedance tantalum capacitor AVX 22

Mouse acidic fibroblast growth factor 1elisa kit detection method fibroblast growth factor (hereinafter referred to as FGF) exists in two closely related forms, namely sputum fibroblast growth factor (bFGF) and acidic fibroblasts Growth factors (abbreviated as aFGF), which have a common receptor on the cell surface, have a wide range of biological effects and are regulated by β-transforming growth factor (hereinafter referred to as TGF-β). Shanghai Jinma Bio Supply Chicken Basic Fibroblast Growth Factor (bFGF) ELISA Kit, Mouse Acidic Fibroblast Growth Factor 1 ELISA Kit, Human Basic Fibroblast Growth Factor 9 (bFGF-9) ELISA Kit, etc. Multiple categories of Elisa kits, please contact us for specific operation detection methods.
The following three horses routine analysis methods:
1. A 12-well cell culture plate was added, and 0.5 ml of a cell culture medium (or 3T3 cells) containing 1 x 104 bovine adrenal vascular endothelial cells (ACE) was added to each well.

2. The sample to be tested was diluted with PBS containing 0.2% gelatin, and 0.5 ml of the diluted sample was added to each well, and the diluted sample of the same concentration was replaced every other day; 0.5 ml of the cell culture solution was added to the control well. Incubate for 4 to 5 days in a saturated water vapor carbon dioxide incubator at 37 ° C in 5% CO 2 .

3. The number of cells grown was counted by MTT method, [3H] deoxythymidine incorporation method or by directly counting cells after trypsin digestion. Dose-response curves were plotted and the highest sample dilution that resulted in a 50% maximal proliferative response was 1 U FGF.

product detailed information

Chinese name:

Mouse acidic fibroblast growth factor

English name: ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)

Product specifications: 48T/96T

Product price: inquiry

Detection range: See Elisa kit instructions for details.

Data processing and results reporting

1 Calculate recovery rate: recovery rate 1 =

2 Calculate the average recovery rate:

Average recovery =

3 Calculate the proportional system error: proportional system error = 100% - average recovery rate

4 Acceptable judgment: The proportional system error is not more than 1/2 of the total error allowed by CLIA 88.